![]() We used the Gibson assembly as a quick and cheap cloning system for introducing sequences into the lentiviral-based pFUGW vector. The development of viral vectors has contributed significantly to the genetic manipulation of endothelial cells. In this work, we describe advanced methods that allow investigating the dynamics of endothelial cell contacts. Advanced microscopic techniques, automation processing, and image analysis software was shown to improve the understanding of vascular biology. ![]() Sticky ends from different BstAPI sites may not be compatible.Endothelial cells of the vascular system are dynamic cells whose molecular adaptability is decisive for the adjustment of homeostasis and organ perfusion. G C A N N N N N T G C C G T N N N N N A C G Sticky ends from different BlpI sites may not be compatible. ![]() Sticky ends from different BtgI sites may not be compatible. PaeR7I does not recognize the sequence CTCTCGAG.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |